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Bacterial strains and plasmids
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Image Search Results


Bacterial strains and plasmids

Journal: Applied and Environmental Microbiology

Article Title: Site-Directed Mutations in the Lanthipeptide Mutacin 1140

doi: 10.1128/AEM.00704-13

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: THY broth and THY agar media were used for antimicrobial activity assays. table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) b Source or reference(s) Strains E. coli DH5α F − φ80lacZΔM15 Δ( lacZYA-argF )U169 recA1 endA1 hsdR17 (rk−, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 tonA Invitrogen M. luteus ATCC 10240 Antibiotic indicator strain ATCC S. aureus ATCC 25923 ATCC S. pneumoniae ATCC 27336 ATCC C. difficile UK1 41 , 42 S. mutans UA159 43 S. mutans ATCC 55676 ATCC JH1140 Wild-type strain 31 JH1140 Phe1Gly mutA ::Phe1Gly site-directed derivative This study JH1140 Phe1Ser mutA ::Phe1Ser site-directed derivative This study JH1140 Phe1Thr mutA ::Phe1Thr site-directed derivative This study JH1140 Phe1Ile mutA ::Phe1Ile site-directed derivative This study JH1140 Phe1Trp mutA ::Phe1Trp site-directed derivative This study JH1140 Trp4Ala mutA ::Trp4Ala site-directed derivative This study JH1140 Trp4insAla mutA ::Trp4insAla site-directed derivative This study JH1140 ΔTrp4 mutA ::ΔTrp4 site-directed derivative This study JH1140 DHA5Ala mutA ::Dha5Ala site-directed derivative This study JH1140 Ala s 7insAla mutA ::Ala s 7insAla site-directed derivative This study JH1140 Arg13Asp mutA ::Arg13Asp site-directed derivative This study JH1140 Phe17insAla mutA ::Phe17insAla site-directed derivative This study JH1140 Asn18Ala mutA ::Asn18Ala site-directed derivative This study JH1140 Trp4Ala-Arg13Asp mutA ::Trp4Ala Arg13Asp site-directed derivative This study JH1140 mut a mutA ::heterodiploid derivatives; Erm r This study Plasmids TOPO-TA Cloning vector; 3′ T overhangs; lacZ for blue/white screening; Kan r , Amp r Invitrogen pVA891 Suicide vector; Erm r 27 pVA891mutA Suicide vector; Erm r ; cloned mutA containing approx 500 bp of flanking DNA Oragenics Inc. Open in a separate window a Site-directed heterodiploid mutants of S. mutans containing two copies of mutA and pVA891 suicide vector. b Erm r , erythromycin resistant; Kan r , kanamycin resistant; Amp r , ampicillin resistant.

Techniques: Plasmid Preparation, Cloning, Clone Assay